期刊
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
卷 89, 期 1, 页码 152-157出版社
SPRINGER
DOI: 10.1007/s00128-012-0648-1
关键词
Cr(VI)-resistance; Tannery effluent; Ochrobactrum anthropi; Cr(VI) reduction; Cell free extract
Chromium-resistant bacteria isolated from industrial wastes can be used to detoxify toxic chromium from contaminated sources. From effluent of Shafiq Tannery, Kasur, Pakistan, bacterial strain STCr-1 that could endure 40 mg mL(-1) of potassium chromate in nutrient agar medium was isolated. STCr-1, identified as Ochrobactrum anthropi by 16S rRNA gene sequence homology, demonstrated substantial Cr(VI) reduction at pH 7 and temperature 37A degrees C. It completely reduced 250 mu g mL(-1) of Cr(VI) and showed 71.2 % Cr(VI) reduction at Cr(VI) concentrations of 550 mu g mL(-1). Rate of Cr(VI) reduction increased with increase in cell and Cr(VI) concentration. The presence of Cu2+, Co2+ and Mn2+ significantly stimulated Cr(VI) reduction. Assay with cell free extracts clearly indicated that Cr(VI) reduction was solely associated with the soluble fraction of the cell.
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