期刊
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
卷 86, 期 1, 页码 114-119出版社
SPRINGER
DOI: 10.1007/s00128-010-0181-z
关键词
Cr(VI) reduction; Bacillus sp; Bioremediation
资金
- Council of Scientific and Industrial Research Government of India [24(0271)/04/EMR-II]
A Cr(VI) reducing bacterial strain FM1 was isolated from heavy metal contaminated agricultural soil irrigated with tannery effluents of Jajmau, Kanpur (India), and was identified as Bacillus sp. on the basis of biochemical methods and 16S rDNA gene sequence analysis. FM1 strain was found to be resistant to some toxic heavy metals (Cr(VI), Cr(III), Cu2+, Co2+, Cd2+, Ni2+ and Zn2+) up to several fold concentrations to the normal levels occurring in highly polluted region. FM1 was resistant to very high concentration of Cr(VI) (1,000 mg/L) and completely reduced 100 mg/L Cr(VI) within 48 h. Factors (pH, temperature, initial Cr(VI) concentration) affecting Cr(VI) reduction under culture condition were also evaluated. Reduction was optimum at 37A degrees C and pH 8. Cr(VI) reduction was enhanced by addition of glucose. The presence of heavy metal cations, such as Cu2+, Co2+, Cd2+, Ni2+ and Zn2+ showed differential effect on reduction. Since strain FM1 could grow in the presence of significant concentrations of metals and due to high Cr(VI) reduction ability, this bacterium may be potentially applicable in Cr(VI) detoxification.
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