期刊
BULLETIN OF ENTOMOLOGICAL RESEARCH
卷 98, 期 6, 页码 613-619出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007485308005932
关键词
chitin synthase gene; RNAi; function; Spodoptera exigua; pest control
类别
资金
- National Basic Research Program of China [IBN-2006CB102001]
- National Natural Science Foundation of China [IBN-30471143]
RNA interference (RNAi) is a powerful tool for rapidly analyzing gene functions. However, little is known about the possible use of dsRNA/siRNA as a pest control method. Here, we demonstrate that dsRNA/siRNA call induce the silence of chitin synthase gene A (CHSA), which is an important gene for the growth and development of cuticles and trachea in beet armyworm, Spodoptera exigua. Based on the in vitro RNAi experiments in an insect cell line (Trichoplusia ni High 5), in vivo RNAi was performed by injecting synthesized dsRNA/siRNA into the 4th instar larvae of S. exigua. Significantly lower levels of CHSA transcripts were detected. In addition, the cuticle of these insects was disordered and the epithelial walls of larval trachea did not expand uniformly in injected individuals. Moreover, Injections significantly increased abnormalities relative to control larvae. These results highlighted the possibility of dsRNA/siRNA for gene function studies in lepidopteran insects and future pest control.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据