期刊
BRITISH POULTRY SCIENCE
卷 55, 期 3, 页码 284-290出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00071668.2014.904501
关键词
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资金
- Fund of China Agriculture Research System [CARS-43]
- Key Research Program of Prospering Agriculture Through Science and Technology of Shanghai Management Office [2009: 2-1]
- Construction Program of Zhedong White Goose Breeding Platform
1. The low-density lipoprotein receptor-related protein 8 (LRP8), a member of the low-density lipoprotein receptor (LDLR) gene family, participates in the supplying of lipid during follicular development. The objective of the study was to identify and characterise the LRP8 gene in goose.2. A 2867 bp fragment that covered the complete coding region (CDS) of goose (Anser cygnoides) LRP8 gene was cloned. It encoded a protein of 917 amino acid residues containing a 24-amino acid signal peptide and 5 functional domains. The goose LRP8 showed high nucleic acid and amino acid identities with those in other species.3. Similarly to duck LRP8 gene, two splice variants of LRP8, LRP8-1 (containing 8 ligand-binding repeats) and LRP8-2 (containing 7 ligand-binding repeats), were identified in goose.4. Semi-quantitative RT-PCR analysis indicates that the LRP8-1 transcript is expressed in heart, liver, spleen, lung, kidney, breast muscle, duodenum, hypothalamus, pituitary and ovary, negligible or absent in sebum and oviduct, and the LRP8-2 transcript is widely expressed in all examined tissues.5. A total of 7 SNPs were identified in the coding region of the goose LRP8 gene.
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