期刊
ANALYTICAL LETTERS
卷 48, 期 7, 页码 1180-1189出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2014.974057
关键词
Ferocactus echidne; Green synthesis; Oligodynamic effect; Silver nanoparticles; Antimicrobial activity
The green synthesis of silver nanoparticles using an aqueous extract of Ferocactus echidne(a member of the cactus family) as a reducing agent is reported. It is simple, efficient, rapid, and ecologically friendly compared to chemical-mediated methods. Ferocactus echidne is a plant of high medicinal value and rich in polyphenolic antioxidants. The extraction is simple and the product rapidly reduces silver ions without involvement of any external chemical agent. The reduction of silver nanoparticles was characterized by ultraviolet-visible spectrometry as a function of time and concentration. The results show that Ferocactus echidne reduces silver ions within 6h depending upon the concentration. Further increases in reaction time may result in a blue shift, indicating an increase in particle size, whereas concentration had a minor effect on the particle size. The structure of synthesized nanoparticles was investigated by infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. The infrared spectra indicated the association of organic materials with silver nanoparticles to serve as capping agents. Scanning electron micrographs showed that synthesized silver nanoparticles were nearly uniform and elliptical in shape with diameters of 20 to 60nm. X-ray diffraction confirmed the formation of silver nanoparticles with an approximate 20nm particle size calculated using the Debye-Scherer equation. Biological tests revealed that the silver nanoparticles were active against gram positive and negative bacteria( Escherichia coli and Staphylococcus aureus) and fungi (Candida albicans), indicating their broad spectrum antibiotic and antifungal abilities.
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