期刊
JOURNAL OF CONTROLLED RELEASE
卷 199, 期 -, 页码 17-28出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2014.12.005
关键词
Autophagic vesicles; Bidirectional accumulation; Anti-tumour; TAT-Beclin 1; HCQ liposomes
资金
- National Natural Science Foundation of China [81373337]
- National Basic Research Program of China (973 Program) [2013CB932504]
- State Key Program of National Natural Science of China [81130060]
Autophagy is a lysosomal degradation pathway that protects cancer cells from multiple endogenous and extraneous stresses, particularly during the pathogenesis of cancer. An autophagic balance exists in the tumour micro-environment. Appropriate disturbance to this balance may have therapeutic potential. Here, we report a novel antitumour strategy based on an autophagic catastrophic vacuolisation effect in tumour cells. We achieved this effect via initiative induction and the terminal restraint of autophagic flux. The TAT-Beclin 1 peptide (T-B) was constructed for the initiative induction of autophagic flux, whereas hydroxychloroquine (HCQ)-loaded liposomes (HCQ-Lip) were constructed for terminal restraint. We demonstrate that T-B, a new CPP tandem autophagy inducing peptide, effectively activates the autophagy signal at the early stage of the autophagy pathway. HCQ deacidified the lysosome during the final stage of autophagy. We combined T-B and HCQ-Lip to induce autophagic catastrophic vacuolisation and death in several tumour cell lines based on the idea of broadening sources of income and reducing expenditure. The co-treated group exhibited at least a 1.86-fold greater and up to 5.66-fold greater cytotoxic effect in vitro. In addition, this strategy showed at least a 2.0-fold tumour inhibitory effect compared to the other groups in vivo. Therefore, this bidirectional accumulation of autophagic vesicles exhibited potential efficacy for tumour treatment. (C) 2014 Elsevier B.V. All rights reserved.
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