期刊
ANALYTICAL LETTERS
卷 49, 期 2, 页码 245-257出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2015.1072824
关键词
Biosensor; immunoassay; nanoparticles; pathogen bacteria
资金
- Act 211 Government of the Russian Federation [02.A03.21.0006]
- RFBR [14-03-01-017A]
This paper reports a novel enzyme-free electrochemical immunoassay for Escherichia coli (ATCC 25922) that involves the formation of an immunocomplex between antibodies immobilized on the surface of a platinum electrode and bacteria-labelled magnetite nanoparticles. Magnetite nanoparticles modified with chitosan were used as the signal label in the immunoassay. The main novelty is the collection of an electrochemical signal from a magnetic nanolabel included within the immunocomplex on the electrode surface. The developed immunosensor is highly specific, enabling the determination of E. coli from 10 to 10(5) colony-forming units (CFU)/mL with the relative standard deviation below 10%. The limit of detection for the immunosensor was 9.3CFU/mL. Bacteria were determined in model mixtures and real samples. The recovery was between 95 and 99%, comparable with results from enzyme-linked immunosorbent assays.
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