期刊
BRITISH JOURNAL OF NUTRITION
卷 107, 期 3, 页码 378-387出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114511003035
关键词
Cocoa flavonoids; Adjuvant arthritis; T-helper lymphocytes; Anti-mycobacterial antibodies
资金
- Ministerio de Educacion y Ciencia, Spain [AGL2005-002823, BES-2006-13640]
- Ministerio de Sanidad y Consumo [CIBER 06/02/0079]
- Generalitat de Catalunya, Spain [SGR 2005-0083]
Cocoa and its flavonoids have potential anti-inflammatory properties in vitro and in acute inflammation models in vivo. The aim of the present study was to ascertain the effects of two cocoa-enriched diets on adjuvant arthritis (AA) in rats, considering not only clinical and biochemical inflammatory indices, but also antibody response and lymphocyte composition. Female Wistar rats were fed with a 5 or 10% cocoa-enriched diet beginning 2 weeks before arthritis induction and until the end of the study. AA was induced by an intradermal injection of heat-killed Mycobacterium butyricum suspension. The hind-paw swelling (plethysmometry), serum anti-mycobacterial antibody concentration (ELISA), blood and inguinal lymph node lymphocyte subset percentage (flow cytometry), and IL-2, interferon gamma and PGE(2) released from splenocytes (ELISA) were assessed. Although the cocoa diets had no significant effect on hind-paw swelling, a tendency to reduce it was observed at the end of the study. Cocoa-enriched diets were able to decrease the serum anti-mycobacterial antibody concentration and the splenocyte PGE2 production, as well as the proportion of T-helper (T-h) lymphocytes in blood and regional lymph nodes, which probably includes cells responsible for the arthritic process. The cocoa diets prevented a decrease in the proportion of regulatory T-cells in blood and a disequilibrium between inguinal lymph node natural killer (NK) CD8(+) and NK CD8(-) subsets. In conclusion, the cocoa-enriched diets during AA were not able to significantly decrease joint inflammation but modified T-h-cell proportions and prevented specific antibody synthesis.
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