期刊
BRITISH JOURNAL OF NUTRITION
卷 100, 期 6, 页码 1251-1259出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114508978284
关键词
Probiotics; Colon carcinogenesis; Apoptosis; Gnotobiotic rats
资金
- Ecologic et Physiologie du Systeme Digestif, Jouy-en-Josas, France [UR910]
- Science et Technologie du Lait et de l'Oeuf, Rennes, France [UMR1253]
Propionibacterium freudenrichii a food-grade bacterium able to kill colon cancer cell lines in vitro by apoptosis, may exert an anticarcinogenic effect in vivo. To assess this hypothesis, we administered daily 2 X 10(10) colony-forming units (CFU) of P. freudenreichii TL133 to human microbiota-associated (HMA) rats For 18 d. Either saline or 1,2-dimethylhydrazine (DMH) was also administered on days 13 and 17 and rats were killed on day 19. The levels of apoptosis and proliferation in the mid and distal colon were assessed by terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick end labelling (TUNEL) and proliferating cell nuclear antigen (PCNA) immunolabelling, respectively. The administration of P.freudenreichii TL133 significantly increased the number of apoptotic cells in DMH-treated rats compared to those given DMH only (P < 0.01). Furthermore, propionibacteria were able to decrease the proliferation index in the distal colon after treatment with DMH (P < 0.01). Conversely, propionibacteria alone did not exert such an effect on healthy colonic mucosa. P. fredenreichii TL133 thus facilitated the elimination of damaged cells by apoptosis in the rat colon after genotoxic insult and may play a protective role against colon cancer.
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