期刊
ANALYTICAL CHEMISTRY
卷 87, 期 3, 页码 1466-1469出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac504110t
关键词
-
资金
- 973 Program [2011CBA00900]
- NSFC [61077064, 60921001]
- Program of International ST Cooperation [2010DFB33880]
- National Major Scientific Instruments and Equipment Development Project [2011YQ03012405]
- GZIELT program [LCY201308]
The application scope of surface plasmon resonance (SPR) and SPR imaging (SPRi) is rapidly growing, and tools such as high-performance and low-cost slides could enable more rapid growth of the field. We describe herein a novel silver slide, addressing the inherent instability of plain silver structure by improving adhesion between the glass substrate and the silver layer with a thin buffer layer of gold. Covered by a self-assembled monolayer (SAM) only, SPR characteristics of the slide remain steady for more than 3 months under regular storage. In a bioassay, the slide substantiates the predicted nearly 100% sensitivity improvement over gold slides and exhibits exceptional performance stability as determined by sensitivity and resolution measurements during the extended 40?000 s multicycle experiment. We demonstrate the suitability of this new slide for large-area SPRi, describing analysis results from a 1?296-ligand protein microarray. We predict this slide structure will provide a stable, high-sensitivity solution for high-throughput SPRi applications and other surface analysis platforms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据