期刊
BRITISH JOURNAL OF HAEMATOLOGY
卷 154, 期 1, 页码 111-121出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2141.2011.08714.x
关键词
Tumour apoptosis; anti-beta(2) mAbs; IgM pentamer; multiple myeloma; haematological and solid tumours
类别
资金
- National Cancer Institute [R01 CA138402, R01 CA138398, P50 CA142509, K99/R00 CA137158]
- Leukemia and Lymphoma Society Translational Research Grants
- Multiple Myeloma Research Foundation
- Commonwealth Foundation for Cancer Research
- International Myeloma Foundation
- Lymphoma Research Foundation
- American Society of Hema-tology
- University Cancer Foundation
- Center for Targeted Therapy of The University of Texas M. D. Anderson Cancer Center
Monoclonal antibodies (mAbs) specific for human beta(2)-microglobulin (beta M-2) have been shown to induce tumour cell apoptosis in haematological and solid tumours via recruiting major histocompatibility complex (MHC) class I molecules into and excluding cytokine receptors from the lipid rafts. Based on these findings, we hypothesized that IgM anti-beta M-2 mAbs might have stronger apoptotic effects because of their pentameric structure. Our results showed that, compared with IgG mAbs, IgM anti-beta M-2 mAbs exhibited stronger tumouricidal activity in vitro against different tumour cells, including myeloma, mantle cell lymphoma, and prostate cancer, and in vivo in a human-like xenografted myeloma mouse model without damaging normal tissues. IgM mAb-induced apoptosis is dependent on the pentameric structure of the mAbs. Disrupting pentameric IgM into monomeric IgM significantly reduced their ability to induce cell apoptosis. Monomeric IgM mAbs were less efficient at recruiting MHC class I molecules into and exclusion of cytokine receptors from lipid rafts, and at activating the intrinsic apoptosis cascade. Thus, we developed and validated the efficacy of anti-beta M-2 IgM mAbs that may be utilized in the clinical setting and showed that IgM anti-beta M-2 mAbs may be more potent than IgG mAbs at inducing tumour apoptosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据