期刊
ANALYTICAL CHEMISTRY
卷 87, 期 8, 页码 4275-4282出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00532
关键词
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资金
- National Science Foundation for Distinguished Young Scholars of China [21325522]
- National Science Foundation for Excellent Youth Scholars of China [21222506, 21422506]
- National Science Foundation of China [91313302, 21205100, 21275122, 21435004]
- National Basic Research Program of China [2013CB933703]
A versatile point-of-care assay platform was developed for simultaneous detection of multiple targets based on a microfluidic paper-based analytic device (mu PAD) using a target-responsive hydrogel to mediate fluidic flow and signal readout. An aptamer-cross-linked hydrogel was used as a target-responsive flow regulator in the mu PAD. In the absence of a target, the hydrogel is formed in the flow channel, stopping the flow in the mu PAD and preventing the colored indicator from traveling to the final observation spot, thus yielding a signal off readout. In contrast, in the presence of a target, no hydrogel is formed because of the preferential interaction of target and aptamer. This allows free fluidic flow in the mu PAD, carrying the indicator to the observation spot and producing a signal on readout. The device is inexpensive to fabricate, easy to use, and disposable after detection. Testing results can be obtained within 6 min by the naked eye via a simple loading operation without the need for any auxiliary equipment. Multiple targets, including cocaine, adenosine, and Pb2+, can be detected simultaneously, even in complex biological matrices such as urine. The reported method offers simple, low cost, rapid, user-friendly, point-of-care testing, which will be useful in many applications.
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