期刊
ANALYTICAL CHEMISTRY
卷 87, 期 7, 页码 3694-3698出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00887
关键词
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资金
- National Basic Research Program [2013CB834703, 2013GB834505, 2011CBA00905, 2009CB930802]
- National Natural Science Foundation of China [21233011, 91123033, 21273252, 21205122, 21261160488, 21072196]
A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30-55 degrees C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.
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