期刊
ANALYTICAL CHEMISTRY
卷 87, 期 3, 页码 1896-1902出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac5040715
关键词
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资金
- Beijing Science and Technology New Star Project [2010B021]
- Scientific Research Foundation for Returned Scholars of Ministry of Education of China
- National Natural Science Foundation of China [21003012, 21273020, 61174010, 21233003]
- Natural Science and Engineering Research Council of Canada
On-site oral fluid testing for drugs of abuse has become prominent in order to take immediate administrative action in an enforcement process. Herein, we report a DVD technology-based indirect competitive immunoassay platform for the quantitative detection of drugs of abuse. A microfluidic approach was adapted to prepare multiplex immunoassays on a standard DVD-R, an unmodified multimode DVD/Blu-Ray drive to read signal, and a free disc-quality analysis software program to process the data. The DVD assay platform was successfully demonstrated for the simultaneous, quantitative detection of drug candidates (morphine and cocaine) in oral fluids with high selectivity. The detection limit achieved was as low as 1.0 ppb for morphine and 5.0 ppb for cocaine, comparable with that of standard mass spectrometry and ELISA methods.
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