期刊
BRITISH JOURNAL OF CANCER
卷 111, 期 8, 页码 1572-1580出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/bjc.2014.454
关键词
microRNA; miR-1246; CCNG2; chemoresistance; cancer stem cell; pancreatic cancer
类别
资金
- Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology
- Third Comprehensive 10-year Strategy for Cancer Control, Ministry of Health, Labor, and Welfare
- Kobayashi Cancer Research Foundation
- Princess Takamatsu Cancer Research Fund, Japan
- National Institute of Biomedical Innovation, Japan
- Grants-in-Aid for Scientific Research [22130009, 26640072] Funding Source: KAKEN
Background: Pancreatic cancer has a poor prognosis because of its high refractoriness to chemotherapy and tumour recurrence, and these properties have been attributed to cancer stem cells (CSCs). MicroRNA (miRNA) regulates various molecular mechanisms of cancer progression associated with CSCs. This study aimed to identify the candidate miRNA and to characterise the clinical significance. Methods: We established gemcitabine-resistant Panc1 cells, and induced CSC-like properties through sphere formation. Candidate miRNAs were selected through microarray analysis. The overexpression and knockdown experiments were performed by evaluating the in vitro cell growth and in vivo tumourigenicity. The expression was studied in 24 pancreatic cancer samples after laser captured microdissection and by immunohistochemical staining. Results: The in vitro drug sensitivity of pancreatic cancer cells was altered according to the miR-1246 expression via CCNG2. In vivo, we found that miR-1246 could increase tumour-initiating potential and induced drug resistance. A high expression level of miR-1246 was correlated with a worse prognosis and CCNG2 expression was significantly lower in those patients. Conclusions: miR-1246 expression was associated with chemoresistance and CSC-like properties via CCNG2, and could predict worse prognosis in pancreatic cancer patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据