期刊
BRITISH JOURNAL OF CANCER
卷 105, 期 1, 页码 65-73出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/bjc.2011.143
关键词
prostate cancer diagnosis; methylation sensitive restriction endonuclease-qPCR; serial testing; primer sequence; methylated and unmethylated sequence; molecular epidemiology; quantitative methylation test
类别
资金
- University of Cincinnati
- Institutional Clinical and Translational Science Award
- NIH/NCRR [1UL1RR026314-01]
- NIEHS [P30-ES006096, 1RC2-ES018758, RC2-ES018789, ES018758, CA112532, R01ES015584]
- [KO7CA138714-01]
- [ES006096]
- [K99ES016817]
BACKGROUND: Prostate-specific antigen (PSA) screening has low specificity. Assessment of methylation status in body fluids may complement PSA screening if the test has high specificity. METHOD: The purpose of this study was to conduct a meta-analysis of the sensitivity and specificity for prostate cancer detection of glutathione-s-transferase-pi (GSTP1) methylation in body fluids (plasma, serum, whole blood, urine, ejaculate, and prostatic secretions). We conducted a comprehensive literature search on Medline (Pubmed). We included studies if they met all four of the following criteria: (1) measurement of DNA methylation in body fluids; (2) a case-control or case-only design; (3) publication in an English journal; and (4) adult subjects. Reviewers conducted data extraction independently using a standardised protocol. Twenty-two studies were finally included in this paper. Primer sequences and methylation method in each study were summarised and evaluated using meta-analyses. This paper represents a unique cross-disciplinary approach to molecular epidemiology. RESULTS: The pooled specificity of GSTP1 promoter methylation measured in plasma, serum, and urine samples from negative-biopsy controls was 0.89 (95% CI, 0.80-0.95). Stratified analyses consistently showed a high specificity across different sample types and methylation methods (include both primer sequences and location). The pooled sensitivity was 0.52 (95% CI, 0.40-0.64). CONCLUSIONS: The pooled specificity of GSTP1 promoter methylation measures in plasma, serum, and urine was excellent and much higher than the specificity of PSA. The sensitivity of GSTP1 was modest, no higher than that of PSA. These results suggest that measurement of GSTP1 promoter methylation in plasma, serum, or urine samples may complement PSA screening for prostate cancer diagnosis. British Journal of Cancer (2011) 105, 65-73. doi:10.1038/bjc.2011.143 www.bjcancer.com Published online 7 June 2011 (C) 2011 Cancer Research UK
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