期刊
BRITISH JOURNAL OF CANCER
卷 104, 期 1, 页码 110-119出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bjc.6605992
关键词
cervical cancer precursors; squamous cervical cancer; diagnostics; marker protein patterns; immunohistochemistry
类别
资金
- Swedish Cancer Foundation [070623, CAN 2007/1044]
- KI [5888/05-722]
- Swedish Research Council [521-2008-2899]
- Medical Research Council
- Cancer Society in Stockholm
- Stockholm County Council
- Swedish Labor Market Insurance
- EU
BACKGROUND: Cytology-based diagnostics of squamous cervical cancer (SCC) precursor lesions is subjective and can be improved by objective markers. METHODS: IHC-based analysis of ANXA6, HSP27, peroxiredoxin 2 (PRDX2), NCF2, and tropomyosin 4 (TPM4) during SCC carcinogenesis. RESULTS: Expression of ANXA6, HSP27, PRDX2, and NCF2 in the cytoplasm of dysplastic cells increased from cervical intraepithelial neoplasia 2/3 (CIN2/3) to microinvasive cancer. Invasive SCC showed lower expression of TPM4 than CIN and normal epithelium. CIN2/3 with the highest sensitivity and specificity differed from normal epithelium by cytoplasmic expression of HSP27. Patients with cytoplasmic HSP27 expression in SCC deviating from that observed in normal epithelium had worse relapse-free (P = 0.019) and overall (P = 0.014) survival. Invasive SCC with the highest sensitivity and specificity differed from normal epithelium by expression of PRDX2 and TPM4 in the cytoplasm, from CIN2/3 by the expression of ANXA6 and TPM4 in the cytoplasm, and from microinvasive SCC by the expression of PRDX2 and ANXA6 in the cytoplasm. The number of sporadic ANXA6 1 cells between the atypical cells increased from CIN2/3 to invasive SCC. CONCLUSION: Detection of expression changes of the proteins ANXA6, HSP27, PRDX2, NCF2, and TPM4 in SCC precursor lesions may aid current cytological and pathological diagnostics and evaluation of prognosis. British Journal of Cancer (2011) 104, 110-119. doi:10.1038/sj.bjc.6605992 www.bjcancer.com Published online 30 November 2010 (C) 2011 Cancer Research UK
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