期刊
BRITISH JOURNAL OF CANCER
卷 101, 期 1, 页码 64-70出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bjc.6605125
关键词
ES cell transplantation; embryonal carcinoma; teratocarcinoma; angiogenesis; vascular corrosion casting
类别
资金
- Spanish Ministry of Education of Science [BFU 2007-66610]
- University of the Basque Country Research Group [GIU08/04]
- University of the Basque Country
- Gangoiti Foundation of Bilbao
BACKGROUND: Carcinoma in situ (CIS) of the testis is considered to be a precancerous germinal cell lesion, but the precise cellular and molecular mechanisms underlying transformation of CIS into invasive pluripotent cancer cells remain to be elucidated. Moreover, a satisfactory animal model for the experimental study of germinal tumours has not been developed to date. METHODS: We have developed a tumour model that involves the microinjection of green fluorescent protein-labelled embryonic stem (ES) cells ( which are functionally equivalent to CIS cells) into syngenic mouse seminiferous tubules, a unique cell microenvironment in which germinal cells mature and CIS arise. To characterise the vascularisation of teratocarcinomas, which arise after cell transplant, we used immunohistochemistry, together with a qualitative and quantitative analysis of scanning electron microscopy images of corrosion casting samples. RESULTS: Embryonic stem cells transplanted into seminiferous tubules did not differentiate into germinal cells, but rather they behaved as invasive embryonal carcinoma (EC) stem cells. The vascular pattern of the experimental teratocarcinomas showed a highly disorganised architecture, and some of the neoplastic capillaries were derived, at least in part, from the original transplanted ES cells. CONCLUSION: The transplantation of pluripotent ES cells into seminiferous tubules efficiently recapitulates the early stages of development of teratocarcinomas. Consequently, this method constitutes a novel in vivo model to study the mechanisms of invasion and progression of experimental germinal tumours. British Journal of Cancer ( 2009) 101, 64-70. doi: 10.1038/sj.bjc.6605125 www.bjcancer.com Published online 9 June 2009 (C) 2009 Cancer Research UK
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