期刊
BRITISH JOURNAL OF CANCER
卷 98, 期 11, 页码 1839-1844出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bjc.6604358
关键词
radiation-induced bystander effects; signalling pathway; mitochondrion; nitric oxide synthase
类别
资金
- NCI NIH HHS [P01 CA049062, CA 49062] Funding Source: Medline
- NIEHS NIH HHS [ES 012888, P30 ES009089, R01 ES012888, ES 09089] Funding Source: Medline
Bystander effects induced by cytoplasmic irradiation have been reported recently. However, the mechanism(s) underlying, such as the functional role of mitochondria, is not clear. In the present study, we used either mtDNA-depleted (rho(0)) A(L) or normal (rho(+)) A(L) cells as irradiated donor cells and normal human skin fibroblasts as receptor cells in a series of medium transfer experiments to investigate the mitochondria-related signal process. Our results indicated that mtDNA-depleted cells or normal AL cells treated with mitochondrial respiratory chain function inhibitors had an attenuated gamma-H2AX induction, which indicates that mitochondria play a functional role in bystander effects. Moreover, it was found that treatment of normal AL donor cells with specific inhibitors of NOS, or inhibitor of mitochondrial calcium uptake (ruthenium red) significantly decreased gamma-H2AX induction and that radiation could stimulate cellular NO and O-2(center dot-) production in irradiated rho(+) A(L) cells, but not in rho(0) A(L) cells. These observations, together with the findings that ruthenium red treatment significantly reduced the NO and O-2(center dot-) levels in irradiated rho(+) A(L) cells, suggest that radiation-induced NO derived from mitochondria might be an intracellular bystander factor and calcium-dependent mitochondrial NOS might play an essential role in the process.
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