期刊
BRIEFINGS IN FUNCTIONAL GENOMICS
卷 9, 期 5-6, 页码 362-373出版社
OXFORD UNIV PRESS
DOI: 10.1093/bfgp/elq023
关键词
transcription factor; DNA binding; binding affinity; ChIP; MITOMI; PBM; SELEX
资金
- Swiss SystemsX.ch initiative
Specific binding of transcription factors (TFs) determines in a large part the connectivity of gene regulatory networks as well as the quantitative level of gene expression. A multiplicity of both experimental and computational methods is currently used to discover and characterize the underlying TF-DNA interactions. Experimental methods can be further subdivided into in vitro-and in vivo-based approaches, each accenting different aspects of TF-binding events. In this review we summarize the flexibility and performance of a selection of both types of experimental methods. In conclusion, we argue that a serial combination of methods with different throughput and data type constitutes an optimal experimental strategy.
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