期刊
BREEDING SCIENCE
卷 64, 期 4, 页码 344-350出版社
JAPANESE SOC BREEDING
DOI: 10.1270/jsbbs.64.344
关键词
Tartary buckwheat; breeding; bitterness; rutinosidase; genetic resources; quality; cross breeding
资金
- Research and Development Projects for Application in Promoting New Policy of Agriculture, Forestry and Fisheries
- Research Project on Development of Agricultural Products and Foods with Health-promoting benefits (NARO), Japan'
Here, we developed a new Tartary buckwheat cultivar 'Manten-Kirari', whose flour contains only trace amounts of rutinosidase and lacked bitterness. The trace-rutinosidase breeding line 'f3g-162' (seed parent), which was obtained from a Nepalese genetic resource, was crossed with Hokkai T8' (pollen parent), the leading variety in Japan, to improve its agronomic characteristics. The obtained progeny were subjected to performance test. 'Manten-Kirari' had no detectable rutinosidase isozymes in an in-gel detection assay and only 1/266 of the rutinosidase activity of 'Hokkai T8'. Dough prepared from 'Manten-Kirari' flour contained almost no hydrolyzed rutin, even 6 11 after the addition of water, whereas the rutin in 'Hokkai T8' dough was completely hydrolyzed within 10 tnin. In a sensory evaluation of the flour from the two varieties, nearly all panelists detected strong bitterness in 'Hokkai T8', whereas no panelists reported bitterness in 'Manten-Kirari'. This is the first report to describe the breeding of a Tartary buckwheat cultivar with reduced rutin hydrolysis and no bitterness in the prepared flour. Notably, the agronomic characteristics of 'Manten-Kirari' were similar to those of 'Hokkai T8', which is the leading variety in Japan. Based on these characteristics, 'Manten-Kirari' is a promising for preparing non-bitter, rutin-rich foods.
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