期刊
ANALYTICAL CHEMISTRY
卷 87, 期 9, 页码 4772-4780出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac5048532
关键词
-
资金
- Funding Program for Next Generation World-Leading Researchers of the Council for Science and Technology Policy (CSTP) [GR066]
- JSPS [26249116, 25889039]
- Japan Society for the Promotion of Science (JSPS)
- GCOE
- Grants-in-Aid for Scientific Research [25889039, 15K14204, 26249116] Funding Source: KAKEN
The dynamic properties of phospholipid (PL) membranes (phase state and phase transition) play crucial roles in biological systems. However, highly sensitive, direct analytical methods that shed light on the nature of lipids and their assemblies have not been developed to date. Here, we describe the analysis of PL-modified Au nanoparticles (Au@ PL) using membrane surface-enhanced Raman spectroscopy (MSERS) and report the properties of the self-assembled PL membranes on the Au nanoparticle. The Raman intensity per PL concentration increased by 50-170 times with Au@PL, as compared to large unilamellar vesicles (LUVs) at the same PL concentration. The phase state and phase transition temperature of the PL membrane of Au@PL were investigated by analyzing the Raman peak ratio (R = I-2882/I-2930). The enhancement at 714 cm(-1) (EF(714)) varied with the hydrocarbon chain length of the PLs and the assembled degree of Au@PLs. In calculation, the EF(714),assembled was estimated to be 111-142 when the distance between AuNPs was 7.0-7.5 nm, which was correlated to the speculative enhancement factor, suggesting that the assembly of the Au@PLs contributed to the MSERS.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据