期刊
TRANSCRIPTION-AUSTIN
卷 1, 期 3, 页码 165-175出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/trns.1.3.13332
关键词
Oct4; PURA; nucleolin; non-coding RNA; pseudogenes
资金
- NIH [R01 HL083473, R01 AI084406]
- NATIONAL CANCER INSTITUTE [R01CA153124, R01CA151574] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL083473] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI084406] Funding Source: NIH RePORTER
Long non-coding RNAs (lncRNAs) have been shown to epigenetically regulate certain genes in human cells. Here we report evidence for the involvement of an antisense lncRNA in the transcriptional regulation of the pluripotency-associated factor Oct4. When an lncRNA antisense to Oct4-pseudogene 5 was suppressed, transcription of Oct4 and Oct4 pseudogenes 4 and 5 was observed to increase. This increase correlated with a loss of silent state epigenetic marks and the histone methyltransferase Ezh2 at the Oct4 promoter. We observed this lncRNA to interact with nucleolin and PURA, a 35 kD single-stranded DNA and RNA binding protein, and found that these proteins may act to negatively regulate this antisense transcript.
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