期刊
JOURNAL OF COMPARATIVE NEUROLOGY
卷 524, 期 1, 页码 199-209出版社
WILEY
DOI: 10.1002/cne.23835
关键词
olfactory sensory neuron; odorant receptor; main olfactory epithelium
资金
- European Research Council [Advanced Grant ORGENECHOICE]
Chemosensory specificity in the main olfactory system of the mouse relies on the expression of approximate to 1,100 odorant receptor (OR) genes across millions of olfactory sensory neurons (OSNs) in the main olfactory epithelium (MOE), and on the coalescence of OSN axons into approximate to 3,600 glomeruli in the olfactory bulb. A traditional approach for visualizing OSNs and their axons consists of tagging an OR gene genetically with an axonal marker that is cotranslated with the OR by virtue of an internal ribosome entry site (IRES). Here we report full cell counts for 15 gene-targeted strains of the OR-IRES-marker design coexpressing a fluorescent protein. These strains represent 11 targeted OR genes, a 1% sample of the OR gene repertoire. We took an empirical, count every cell strategy: we counted all fluorescent cell profiles with a nuclear profile within the cytoplasm, on all serial coronal sections under a confocal microscope, a total of 685,673 cells in 56 mice at postnatal day 21. We then applied a strain-specific Abercrombie correction to these OSN counts in order to obtain a closer approximation of the true OSN numbers. We found a 17-fold range in the average (corrected) OSN number across these 11 OR genes. In the same series of coronal sections, we then determined the total volume of the glomeruli (TGV) formed by coalescence of the fluorescent axons. We found a strong linear correlation between OSN number and TGV, suggesting that TGV can be used as a surrogate measurement for estimating OSN numbers in these gene-targeted strains. J. Comp. Neurol. 524:199-209, 2016. (c) 2015 Wiley Periodicals, Inc.
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