期刊
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
卷 45, 期 4, 页码 693-700出版社
UNIV SAO PAULO, CONJUNTO QUIMICAS
DOI: 10.1590/S1984-82502009000400012
关键词
Fluconazole/quantitative determination; Microbiological assay/agar diffusion; High performance liquid chromatography
The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r(2)=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry laboratories.
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