期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 49, 期 -, 页码 93-100出版社
SPRINGER
DOI: 10.1016/j.bjm.2018.06.004
关键词
Cattle; Antimicrobial susceptibility; Klebsiella pneumonia; MLST; Virulence genes
类别
资金
- Fundamental Research Funds for the Central Universities [XDJK2017D083]
- Innovation Project for the Social Undertakings and People's Livelihood Protection in Chongqing [CSTC2015SHMSZX80020]
- Frontiers and basic research projects in Chongqing [cstc2016jcyA0235]
Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93.4% of the strains. Of which, TEM was the most prevalent (93.4%), followed by CTX-M and SHV were 57.6% and 39.4%, respectively. A main mutation pattern of quinoloneresistance-determining region, Thr83-Ieu and Asp87-Asn in gyrA and Ser87-Ile in parC, was detected in 33 K. pneumoniae isolates. All the isolates harbored at least two virulence factor genes, with ureA (97.0%) and wabG (91.0%) exhibiting high carriage rates in 33 K. pneumoniae isolates. MLST revealed 7 sequence types, of which 3 STs (2541, 2581 and 2844) were newly assigned. Using eBURST, ST2844 and ST2541 were assigned to new clonal complex 2844. Our study provides evidence and biological characteristics of K. pneumoniae isolates from cattle upper respiratory tract in Southwest China. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
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