期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 45, 期 2, 页码 395-402出版社
SOC BRASILEIRA MICROBIOLOGIA
DOI: 10.1590/S1517-83822014000200005
关键词
biopolymers; microbial community; polyhydroxyalkanoates; RISA; Thauera sp.
类别
资金
- Polish Ministry of Science and Higher Education [N N523 420237]
Polyhydroxyalkanoates (PHAs) can be produced by microorganisms and are a biodegradable alternative to fossil-fuel based plastics. Currently, the focus is on reducing production costs by exploring alternative substrates for PHAs production, and on producing copolymers which are less brittle than monomers. Accordingly, this study used a substrate consisting of wastewater from waste-glycerol fermentation, supplemented with different amounts of acetic and propionic acids. These substrates were used to feed mixed microbial communities enriched from activated sludge in a sequencing batch reactor. A reactor supplemented with 2 mL of acetic acid produced 227.8 mg/L of a homopolymer of hydroxybutyrate (3HB); 4mL of acetic acid produced 279.8 mg/L 3HB; whereas 4mL of propionic acid produced 673.0 mg/L of a copolymer of 3HB and 3HV (hydroxyvalerate). Ribosomal Intergenic Spacer Analysis (RISA) was used to show the differences between the communities created in the reactors. Thauera species predominated in biomass that produced 3HB; Paracoccus denitrificans in the biomass that produced 3HB-co-3HV. Because P. denitrificans produced the more desirable copolymer, it may be advantageous to promote its growth in PHAs-producing reactors by adding propionate.
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