期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 42, 期 1, 页码 66-74出版社
SPRINGER
DOI: 10.1590/S1517-83822011000100009
关键词
Copper contamination; vineyard soil; mining waste; copper biosorption; bioremediation
类别
资金
- Brazilian National Research Council (CNPq)
- Auburn University at Montgomery
Vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. Bioremediation is an efficient process for the treatment of contaminated sites. Efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. In this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. Eighty isolates (45 from vineyard Mollisol, 35 from Inceptisol) were obtained from EMBRAPA (Empresa Brasileira de Pesquisa Agropecuaria) experimental station, Bento Goncalves, RS, Brazil (29 degrees 09'53.92 '' S and 51 degrees 31'39.40 '' W) and 26 were obtained from copper mining waste from Cacapava do Sul, RS, Brazil (30 degrees 29'43.48 '' S and 53'32'37.87W). Based on resistance to copper toxicity and biosorption, 15 isolates were identified by 16S rRNA gene sequencing. Maximal copper resistance and biosorption at high copper concentration were observed with isolate N2 which removed 80 mg L-1 in 24 h. Contrarily isolate N11 (Bacillus pumilus) displayed the highest specific copper biosorption (121.82 mg/L/OD unit in 24 h). GenBank MEGABLAST analysis revealed that isolate N2 is 99% similar to Staphylococcus pasteuri. Results indicate that several of our isolates have potential use for bioremediation treatment of vineyards soils and mining waste contaminated with high copper concentration.
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