期刊
BRAZILIAN JOURNAL OF INFECTIOUS DISEASES
卷 18, 期 6, 页码 672-674出版社
CONTEXTO
DOI: 10.1016/j.bjid.2014.08.004
关键词
Multiplex-PCR; Clostridium difficile; HRM; Toxin gene profiling
Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study. (C) 2014 Elsevier Editora Ltda. All rights reserved.
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