期刊
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
卷 29, 期 2, 页码 325-335出版社
SPRINGER HEIDELBERG
DOI: 10.1590/S0104-66322012000200013
关键词
Casein hydrolysate; Liposomes; Lyophilized liposomes; Microencapsulation
资金
- CNPq - Conselho Nacional de Pesquisa e Desenvolvimento (Brazil)
A commercial casein hydrolysate was microencapsulated in liposomes produced with non-purified soy lecithin, cryoprotected with two different disaccharides and lyophilized. The encapsulation efficiency of casein hydrolysate ranged from 30 to 40%. The powders were analyzed by differential scanning calorimetry (DSC), scanning electron micrography (SEM), infrared spectroscopy (FTIR) and wide angle X-ray diffraction (WAXD). DSC data revealed the presence of an exothermal transition in empty lyophilized liposomes, which was ascribed to the presence of a quasicrystalline lamellar phase (intermediary characteristics between the L-beta and L-c phases). The addition of peptides to the liposomal system caused the disappearance of this exothermic phenomenon, as they were located in the polar headgroup portion of the bilayer, causing disorder and preventing the formation of the quasicrystalline phase. Infrared data indicated the presence of the peptides in the lyophilized formulations and showed that the cryoprotectants interacted effectively with the polar heads of phospholipids in the bilayer.
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