期刊
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
卷 27, 期 4, 页码 499-506出版社
BRAZILIAN SOC CHEMICAL ENG
DOI: 10.1590/S0104-66322010000400001
关键词
Clavulanic acid; Streptomyces clavuligerus; Glycerol; Ornithine; Fed-batch
资金
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [03/11722-5, 05/55079-4]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [474295/2009-5]
The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp-max) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp(max)) of 13.7 mg.L-1. h(-1) was obtained with 0.66 g.L-1 of ornithine, 44.2% higher than the Pp(max) in the control run. In fed-batch experiments, Cp-max varied within the narrow range from 1.254 to 1.405 g.L-1, 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp(max), although it influenced only slightly the Cp-max. Concerning glycerol, the highest CA production of 1.6 g.L-1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L-1) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6: 1), on CA biosynthesis.
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