期刊
BRAZILIAN JOURNAL OF BIOLOGY
卷 70, 期 4, 页码 1217-1222出版社
INT INST ECOLOGY
DOI: 10.1590/S1519-69842010000600011
关键词
genotoxicity; river pollution; biomarkers; metals
类别
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [477192/2007-6]
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS)
The Sinos River, in southern Brazil, is polluted by industrial discharges and untreated urban wastes. Fish genotoxicity biomarkers are valuable parameters for environmental risk assessment. In this study, we used the comet assay to detect genotoxicity due to multiple sources of pollution in the peripheral blood of a native fish species (Hyphessobrycon luetkenii). In addition, we analysed possible DNA damage from aluminum, lead, chromium, copper, nickel, iron and zinc contamination. Water samples were collected seasonally from three sampling sites and the fish were assessed under laboratory conditions. Water chemical analysis showed an increased level of aluminum and iron in most of the samples at sites 2 and 3, located in the middle and lower river course, respectively. The index of DNA damage assessed by the comet assay demonstrated no significant differences in different seasons or at the different sampling sites, while the frequency of cells with DNA damage was higher in water samples collected at sites 1 and 2 during the spring season. None of the metals studied seems to be associated with the increase in the frequency of cells with DNA damage observed during the spring season. The results of this study indicate that the Sinos River is contaminated with substances that are genotoxic to fish, including the waters near the river spring.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据