期刊
BRAIN STRUCTURE & FUNCTION
卷 220, 期 3, 页码 1369-1379出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00429-014-0730-z
关键词
Neuronal morphology; Connectivity; Sparse labeling; Circuit reconstruction; Neuron-type classification; Alzheimer's disease
资金
- European Commission
- INSERM
- PDBEB Programm CNC Coimbra
- LabEx BRAIN
- Conseil de la Region d'Aquitaine
- ANR [10-MALZ-0009]
- DFG [SFB 870]
- Bernstein Center for Computational Neuroscience in Tuebingen - German Federal Ministry of Education and Research (BMBF) [FKZ: 01GQ1002]
- Max Planck Institute for Biological Cybernetics, Tuebingen
- Max Planck Florida Institute for Neuroscience, Jupiter
- Werner Reichardt Center for Integrative Neuroscience, Tuebingen
Glycoprotein-deleted rabies virus (RABV a dagger G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV a dagger G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV a dagger G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (> 14 months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV a dagger G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits.
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