期刊
BRAIN STRUCTURE & FUNCTION
卷 221, 期 2, 页码 1157-1172出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00429-014-0962-y
关键词
Muscarinic acetylcholine receptors; Microglia; Brain macrophages; Stroke; Alzheimer's disease; Glioma; Multiple sclerosis
资金
- Deutsche Forschungsgemeinschaft [SFB-TRR43]
- Neurocure
- Charite Universitatsmedizin Berlin
- Berlin Institute of Health
Microglia undergo a process of activation in pathology which is controlled by many factors including neurotransmitters. We found that a subpopulation (11 %) of freshly isolated adult microglia respond to the muscarinic acetylcholine receptor agonist carbachol with a Ca2+ increase and a subpopulation of similar size (16 %) was observed by FACS analysis using an antibody against the M3 receptor subtype. The carbachol-sensitive population increased in microglia/brain macrophages isolated from tissue of mouse models for stroke (60 %) and Alzheimer's disease (25 %), but not for glioma and multiple sclerosis. Microglia cultured from adult and neonatal brain contained a carbachol-sensitive subpopulation (8 and 9 %), which was increased by treatment with interferon-gamma to around 60 %. This increase was sensitive to blockers of protein synthesis and correlated with an upregulation of the M3 receptor subtype and with an increased expression of MHC-I and MHC-II. Carbachol was a chemoattractant for microglia and decreased their phagocytic activity.
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