期刊
BRAIN RESEARCH BULLETIN
卷 86, 期 3-4, 页码 246-253出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2011.08.006
关键词
Vitamin A supplementation; Low doses; Female rats; Central nervous system; Nitrosative stress; Respiratory chain activity
资金
- Conselho Nacional de Pesquisa e Desenvolvimento Tecnologico (CNPq)
- Rede Instituto Brasileiro de Neurociencia (IBN-Net) [01.06.0842-00]
Vitamin A supplementation among women is a common habit worldwide in an attempt to slow aging progression due to the antioxidant potential attributed to retinoids. Nonetheless, vitamin A elicits a myriad of side effects that result from either therapeutic or inadvertent intake at varying doses for different periods. The mechanism behind such effects remains to be elucidated. In this regard, we performed the present work aiming to investigate the effects of vitamin A supplementation at 100, 200, or 500 IU/kg day(-1) for 2 months on female rat brain, analyzing tissue lipid peroxidation levels, antioxidant enzyme activities (both Cu/Zn-superoxide dismutase - SOD - and Mn-SOD); glutathione S-transferase (GST) and monoamine oxidase (MAO) enzyme activity; mitochondrial respiratory chain activity and redox parameters in mitochondrial membranes, as well as quantifying alpha- and beta-synucleins, beta-amyloid peptide(1-40), immunoglobulin heavy-chain binding protein/78 kDa glucose-regulated protein (BiP/GRP78), receptor for advanced glycation end products (RAGE). D2 receptor, and tumor necrosis factor-alpha (INF-alpha) contents in rat frontal cortex, hippocampus, striatum, and cerebellum. We observed increased lipid peroxidation marker levels, altered Cu/Zn-SOD and Mn-SOD enzyme activities, mitochondrial nitrosative stress, and impaired respiratory chain activity in such brain regions. On the other hand, we did not find any change in MAO and GST enzyme activities, and on alpha- and beta-synucleins, beta-amyloid peptide(1-40), GRP78/BiP, RAGE, D2 receptor, and TNF-alpha contents. Importantly, we did not observed any evidence regarding an antioxidant effect of such vitamin at low doses in this experimental model. The use of vitamin A as an antioxidant therapy among women needs to be reexamined. (C) 2011 Elsevier Inc. All rights reserved.
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