4.5 Article

α-Synuclein overexpression during manganese-induced apoptosis in SH-SY5Y neuroblastoma cells

期刊

BRAIN RESEARCH BULLETIN
卷 81, 期 4-5, 页码 428-433

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2009.11.007

关键词

alpha-Synuclein; Apoptosis; Manganese; SH-SY5Y cell

资金

  1. National Natural Science Foundation of China [30800898]
  2. China Medical University

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Chronic inorganic manganese (Mn) exposure has been known to induce neurological disorders similar to Parkinson's disease (PD). Apoptosis has been shown to be involved in manganese-induced neurotoxicity. However, the up-stream molecular mechanisms for cell apoptosis are not established. a-Synuclein (alpha-syn) is a major component of intracellular inclusions in PD, Alzheimer's disease (AD), and other neurodegenerative disorders. We investigated the role of alpha-syn in manganese chloride (MnCl2)-induced apoptosis. Results show that MnCl2 enhanced transcriptional and translational alpha-syn overexpression, and apoptosis as measured by caspase-3 activity and flow cytometry. Overexpressing alpha-syn exacerbated manganese-induced apoptosis, whereas antisense alpha-syn treatment significantly reversed MnCl2-induced apoptosis in human neuroblastoma SH-SY5Y cells. In conclusion, our results imply that intracellular alpha-syn overexpression may be responsible for MnCl2-induced apoptosis. (C) 2009 Elsevier Inc. All rights reserved.

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