期刊
BRAIN RESEARCH
卷 1371, 期 -, 页码 129-139出版社
ELSEVIER
DOI: 10.1016/j.brainres.2010.10.088
关键词
Endothelial vascular growth factor (VEGF); Traumatic brain injury (TBI); Marrow stromal cell; Collagen scaffold; Restorative therapy
资金
- National Institutes of Health [PO1 NS42345, RO1 NS042259, P41 EB002520]
We have previously demonstrated that human marrow stromal cells (hMSCs) embedded in collagen I scaffolds significantly enhance the restorative therapeutic effect of hMSCs after traumatic brain injury (TBI). In this study, we test the hypothesis that the collagen scaffold alters gene expression in hMSCs and that hMSCs impregnated into scaffolds increase the astrocytic expression of vascular endothelial growth factor (VEGF) in the injured brain. Following TBI induced by controlled cortical impact injury, scaffold with hMSCs (3.0 x 10(6)), hMSCs-only and saline were implanted into the lesion cavity one week after brain injury (n = 8/each group). Morris water maze and modified neurological severity scores were performed to evaluate the spatial learning and sensorimotor functions, respectively. Lesion volume and expression of VEGF were measured one week after different treatments. In vitro, total RNA from hMSCs was extracted one week after culture with or without collagen I scaffold for evaluation of gene microarrays. Furthermore, an RT-PCR study on a select subgroup of genes was performed to identify the changes of expression between the culturing hMSCs with collagen scaffolds and hMSCs only. The treatment of TBI with collagen scaffold impregnated with hMSCs significantly decreases the functional deficits from TB! within 7 days after treatment, and significantly enhances the VEGF expression of astrocytes in the injured brain compared to the hMSCs-only group. In vitro data indicate that collagen scaffolds stimulate hMSCs to express multiple factors which may contribute to hMSC survival, tissue repair and functional recovery after TBI. (C) 2010 Elsevier B.V. All rights reserved.
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