期刊
BRAIN RESEARCH
卷 1329, 期 -, 页码 45-54出版社
ELSEVIER
DOI: 10.1016/j.brainres.2010.03.012
关键词
Astroglia; Microglia; Dexamethasone; Lipopolysaccharide; Experimental bacterial meningitis
资金
- Deutsche Forschungsgemeinschaft DFG [De 292/11]
- Graduiertenkolleg 723 Development and Plasticity of the Nervous System
We analyzed the effect of dexamethasone on gram-negative bacteria derived lipopolysaccharide (LPS) induced inflammation in astroglial/microglial co-cultures. At the cellular level the microglial phenotype converted to an activated type after LPS incubation. Furthermore, LPS compromised functional astioglial properties like membrane resting potential, intracellular coupling and connexin 43 (Cx43) expression. This change in Cx43 expression was not due to a down-regulation of Cx43 mRNA expression. Morphological and functional changes were accompanied by a timedependent release of inflammation related cytokines. Co-incubation of dexamethasone with LPS prevented these LPS-induced changes within our glial co-culture model. The ability of dexamethasone to reconstitute astrocytic properties and to decrease microglial activation in vitro could be one possible explanation for the beneficial effects of dexamethasone in the treatment of acute bacterial meningitis in vivo. (C) 2010 Elsevier By. All rights reserved.
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