期刊
BRAIN RESEARCH
卷 1308, 期 -, 页码 153-157出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2009.10.019
关键词
Ca(2+) channel; L-type; R-type; Protein; beta(3); alpha 2 delta; Audiogenic seizure
资金
- National Institute Health [NS047193, AA11284, AA11628]
The inferior colliculus (IC) is the consensus site for seizure initiation in the genetically epilepsy-prone rat (GEPR). We have previously reported that the current density of high threshold voltage-activated (HVA) calcium (Ca(2+)) channels was markedly enhanced in IC neurons of the GEPR-3 (moderate seizure severity substrain of the GEPR). The present study examines whether subunit protein levels of HVA Ca(2+) channels are altered in IC neurons that exhibit enhanced Ca(2+) current density. Quantification shows that the levels of protein expression of the Ca(2+) channel pore-forming alpha 1D (L-type) and alpha 1E subunits (R-type) were significantly increased in IC neurons of seizure-naive GEPR-3s (SN-GEPR-3s) compared to control Sprague-Dawley (SD) rats. Significant increases and decreases in the levels of protein expression of Ca(2+) channel regulatory beta 3 and alpha 2 delta subunits occurred in IC neurons of SN-GEPR-3s compared to control SD rats, respectively. No changes occurred in the protein expression of Ca(2+) channel pore-forming alpha 1A (P/Q-type), alpha 1B (N-type) and alpha 1C (L-type) subunits in IC neurons of SN-GEPR-3s compared to control SD rats. A single seizure selectively enhanced protein expression of Ca(2+) channel alpha 1A subunits in IC neurons of GEPR-3s. Thus, up-regulation of Ca(2+) channel alpha 1D and alpha 1E subunits may represent the molecular mechanisms for the enhanced current density of L- and R-type of HVA Ca(2+) channels in IC neurons of the GEPR, and may contribute to the genetic basis of their enhanced seizure susceptibility. The up-regulation of Ca(2+) channel alpha 1A subunits induced by seizures may contribute to the increasing IC neuronal excitability that results from repetitive seizures in the GEPR. (C) 2009 Elsevier B.V. All rights reserved.
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