期刊
ANALYTICAL CHEMISTRY
卷 87, 期 4, 页码 2282-2289出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac503974e
关键词
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资金
- National Natural Science Foundation of China [31327001, 31470220]
- Chinese Academy of Sciences [YZ201236, KSZD-EW-Z-021-1-5]
- Natural Environment Research Council [NE/M002934/1] Funding Source: researchfish
- NERC [NE/M002934/1] Funding Source: UKRI
Raman-activated cell sorting (RACS) is a promising single-cell technology that holds several significant advantages, as RACS is label-free, information-rich, and potentially in situ. To date, the ability of the technique to identify single cells in a high-speed flow has been limited by inherent weakness of the spontaneous Raman signal. Here we present an alternative pause-and-sort RACS microfluidic system that combines positive dielectrophoresis (pDEP) for single-cell trap and release with a solenoid-valve-suction-based switch for cell separation. This has allowed the integration of trapping, Raman identification, and automatic separation of individual cells in a high-speed flow. By exerting a periodical pDEP field, single cells were trapped, ordered, and positioned individually to the detection point for Raman measurement. As a proof-of-concept demonstration, a mixture of two cell strains containing carotenoid-producing yeast (9%) and non-carotenoid-producing Saccharomyces cerevisiae (91%) was sorted, which enriched the former to 73% on average and showed a fast Raman-activated cell sorting at the subsecond level.
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