期刊
BRAIN RESEARCH
卷 1249, 期 -, 页码 79-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2008.10.033
关键词
GABAergic boutons; Post-tetanic potentiation; Cultured hippocampal neurons; Ca2+-imaging; L-type Ca2+-channels; Isradipine
资金
- Danish Medical Research Council
- University of Aarhus Research Fund
We have previously demonstrated that L-type Ca2+ channels are involved in post-tetanic potentiation (PTP) of GABAergic IPSCs in cultured hippocampal neurons. Here we have used intracellular Fluo-3 to detect [Ca2+](i) in single GABAergic boutons in response to stimulation that evokes PTP. During control stimulation of the presynaptic GABAergic neuron at 40 Hz for 1-2 s, Delta F/F-0 increased rapidly to a peak value and started to decline shortly after the train ended, returning to baseline within 10-20 s. The L-type channel blocker, isradipine (5 mu M), had no significant effect on the amplitude or kinetics of the Ca2+ signal. Following blockade of N- and P/Q-type Ca2+-channels, the amplitude was reduced by 52.9 +/- 3%. Isradipine caused a reduction of the remaining response (by 26.6 +/- 5%, P<0.01), that was fully reversible on washing. The L-type channel agonist, BayK 8644 (8 mu M), caused a significant enhancement of the peak (by 18.7%+/- 7%, P <0.05). The rising phase of the Ca2+ signal, which is related to the rate of entry of Ca2+ into the bouton, was decreased by isradipine (by 25.5 +/- 6%, P<0.05) and enhanced by BayK 8644 (by 45.2% 16%, P<0.05). These Ca2+ imaging experiments support the putative role of L-type channels in PTP of GABAergic synapses on cultured hippocampal. neurons. We expect L-channels to be few in number, although they may couple strongly to intracellular signalling cascades that could amplify a signal that regulates synaptic vesicle turnover in the GABAergic boutons. (C) 2008 Elsevier B.V. All rights reserved.
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