期刊
BRAIN RESEARCH
卷 1201, 期 -, 页码 88-92出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2008.01.065
关键词
nucleus ambiguus; parasympathetic; ATP; purinergic; glutamate release
资金
- NHLBI NIH HHS [R01 HL049965, R01 HL049965-16, R01 HL059895, R01 HL072006, HL49965] Funding Source: Medline
Recent work has shown that adenosine 5'-triphosphate (ATP) plays an important role in modulating the activity of parasympathetic cardiac vagal neurons that dominate the neural control of heart rate. This study examined the mechanisms by which activation of ATP receptors modulates excitatory neurotransmission to cardiac vagal neurons. Glutamatergic activity to cardiac vagal neurons was isolated and examined using whole-cell patch-clamp recordings in an in vitro brain slice preparation in rats. ATP (100 mu M) evoked increases in the frequency of glutamatergic miniature excitatory postsynaptic currents (mEPSCs) in cardiac vagal neurons which were blocked by the broad P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic add (PPADS, 100 mu M. Application of the selective P2X receptor agonist, alpha, beta-methylene ATP (100 mu M), also increased glutamatergic mEPSCs neurotransmission to cardiac vagal neurons indicating P2X receptors enhance glutamatergic release to cardiac vagal neurons. The evoked increase in glutamatergic mEPSC was unaltered by the voltage-gated calcium channel blocker cadmium, and was abolished by the selective P2X receptor antagonist 2',3'-O-(2,4,6-Trinitrophenyl) adenosine 5'-triphosphate, TNP-ATP (100 mu M). This work demonstrates that the ATP evoked facilitation of excitatory neurotransmission to cardiac vagal neurons is dependent upon activation of P2X receptors on glutamatergic presynaptic terminals. (C) 2008 Elsevier B.V. All rights reserved.
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