期刊
BRAIN RESEARCH
卷 1188, 期 -, 页码 9-16出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2007.10.045
关键词
Complex I; pyruvate dehydrogenase; Parkinson's disease; down syndrome
Mitochondrial function in the brain of mouse trisomy 16, an animal model of Down syndrome with accelerated neuron death, was studied in isolated cortex mitochondria. Using an oxygen-sensitive Clarke electrode, a selective 16% decrease in respiration was detected with the Complex I substrates malate and glutamate but not with the Complex II substrate succinate. Western blotting revealed a 20% decrease in the 20 kDa subunit of Complex I in Ts16 brain cortex homogenates with no significant decrease in marker proteins for the other complexes of the electron transport chain. Although no differences in H2O2 production or maximal calcium uptake were detected in the Ts16 mitochondria, there was an 18% decrease in pyruvate dehydrogenase levels, a change associated with oxidative stress in ischemia. These results are similar to those found in Parkinson's disease suggesting some neuro degenerative diseases may have mitochondrial pathology as a common step. (c) 2007 Elsevier B.V. All rights reserved.
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