期刊
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
卷 300, 期 1, 页码 F24-F30出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00347.2010
关键词
capillary permeability; Ficoll; glomerular filtration; glomerular sieving coefficient
资金
- Swedish Medical Research Council [08285]
- Heart and Lung Foundation
- Medical Faculty at Lund University
Axelsson J, Rippe A, Rippe B. Transient and sustained increases in glomerular permeability following ANP infusion in rats. Am J Physiol Renal Physiol 300: F24-F30, 2011. First published October 13, 2010; doi:10.1152/ajprenal.00347.2010.-The present study was performed to investigate the effects of systemic atrial natriuretic peptide (ANP) infusion on the glomerular permeability to macromolecules in rats. In anesthetized Wistar rats (250-280 g), the left urether was cannulated for urine collection while simultaneously blood access was achieved. Rats were continuously infused intravenously with ANP [30 ng.kg(-1).min(-1) (Lo-ANP; n = 8) or 800 ng.kg(-1).min(-1) (Hi-ANP; n = 10)] or 0.9% NaCl (SHAM; n =16), respectively, and with polydisperse FITC-Ficoll-70/400 (molecular radius 13-90 angstrom) and Cr-51-EDTA for 2 h. Plasma and urine samples were taken at 5, 15, 30, 60, and 120 min of ANP infusion and analyzed by high-performance size-exclusion chromatography (HPLC) for determination of glomerular sieving coefficients (theta) for Ficoll. GFR was also assessed (51Cr-EDTA). In Hi-ANP, there was a rapid (within 5 min), but bimodal, increase in glomerular permeability. theta to high-molecular-weight Ficoll thus reached a maximum at 15 min, after which theta returned to near control at 30 min, to again increase moderately at 60 and 120 min. In Lo-ANP, there was also a rapid, reversible increase in glomerular theta, returning to near control at 30 min, followed by just a tendency of a sustained increase in permeability, but with a significant increase in large-pore radius. In conclusion, in Hi-ANP there was a rapid increase in glomerular permeability, with an early, partly reversible permeability peak, followed by a (moderate) sustained increase in permeability. In Lo-ANP animals, only the initial permeability peak was evident. In both Lo-ANP and Hi-ANP, the glomerular sieving pattern observed was found to mainly reflect an increase in the number and radius of large pores in the glomerular filter.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据