期刊
BRAIN BEHAVIOR AND IMMUNITY
卷 23, 期 7, 页码 1038-1052出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2009.06.004
关键词
Area postrema; Central autonomic system; Cyclooxygenase-2; Endothelial cells; Lipopolysaccharide; Perivascular cells; Staphylococcal enterotoxin B; Vagus nerve
资金
- Spanish Ministry of Education and Science
- Carlos III Health Institute
- [NS-21182]
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R37NS021182, R01NS021182] Funding Source: NIH RePORTER
Bacterial lipopolysaccharide (LPS) is widely used to study immune influences on the CNS, and cerebrovascular prostaglandin (PG) synthesis is implicated in mediating LPS influences on some acute phase responses. Other bacterial products, such as staphylococcal enterotoxin B (SEB), impact target tissues differently in that their effects are T-lymphocyte-dependent, yet both LPS and SEB recruit a partially overlapping set of subcortical central autonomic cell groups. We sought to compare neurovascular responses to the two pathogens, and the mechanisms by which they may access the brain. Rats received iv injections of LPS (2 mu g/kg), SEB (1 mg/kg) or vehicle and were sacrificed 0.5-3 h later. Both challenges engaged vascular cells as early 0.5 h, as evidenced by induced expression of the vascular early response gene (Verge), and the immediate-early gene, NGFI-B. Cyclooxygenase-2 (COX-2) expression was detected in both endothelial and perivascular cells (PVCs) in response to LPS, but only in PVCs of SEB-challenged animals. The non-selective COX inhibitor, indomethacin (1 mg/kg, iv), blocked LPS-induced activation in a subset of central autonomic structures, but failed to alter SEB-driven responses. Liposome mediated ablation of PVCs modulated the CNS response to LPS, did not affect the SEB-induced activational profile. By contrast, disruptions of interoceptive signaling by area postrema lesions or vagotomy (complete or hepatic) markedly attenuated SEB-, but not LPS-, stimulated central activational responses. Despite partial overlap in their neuronal and vascular response profiles, LPS and SEB appear to use distinct mechanisms to access the brain. (C) 2009 Elsevier Inc. All rights reserved.
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