期刊
BRAIN BEHAVIOR AND IMMUNITY
卷 22, 期 3, 页码 301-311出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2007.08.014
关键词
aging; hippocampus; interleukin-1; laser capture microdissection; lipopolysaccharide; working memory
资金
- NIA NIH HHS [R01 AG016710-07, R01 AG016710, R01 AG023580, AG16710, AG023580] Funding Source: Medline
- NIDDK NIH HHS [R01 DK064862, DK64862] Funding Source: Medline
- NIMH NIH HHS [R01 MH069148-05, R01 MH069148, MH069148] Funding Source: Medline
Acute cognitive disorders are common in elderly patients with peripheral infections but it is not clear why. Here, we injected old and Young mice with Escherichia coli lipopolysaccharide (LPS) to mimic an acute peripheral infection and separated the hippocampal neuronal cell layers from the surrounding hippocampal tissue by laser capture microdissection and measured mRNA for several inflammatory cytokines (IL-1 beta, IL-6, and TNF alpha) that are known to disrupt cognition. The results showed that old mice had an increased inflammatory response in the hippocampus after LPS compared to younger cohorts. Immunohistochemistry further showed more microglial cells in the hippocampus of old mice compared to young adults, and that more IL-1 beta-positive cells were present in the dentate gyrus and in the CA1, CA2, and CA3 regions of LPS-treated old mice compared to young adults. In a test of cognition that required animals to effectively integrate new information with a preexisting schema to complete a spatial task, we found that hippocampal processing is more easily disrupted in old animals than in younger ones when the peripheral innate immune system is stimulated. Collectively, the results suggest that aging can facilitate neurobehavioral complications associated with peripheral infections probably by allowing the over expression of inflammatory cytokines in brain areas that mediate cognitive processing. (c) 2007 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据