期刊
JOURNAL OF CLINICAL VIROLOGY
卷 69, 期 -, 页码 81-85出版社
ELSEVIER
DOI: 10.1016/j.jcv.2015.05.022
关键词
MERS-CoV; Real-time RT-PCR; EQA; QPCR; Molecular; Diagnosis; Viral load; Quality control
类别
资金
- European Center for Disease Prevention and Control (ECDC) by supporting the European Network for Diagnostics of 'Imported' Viral Diseases (ENIVD) [1 ECD.4221-FW/ECDC/2013/012]
Background: Since the discovery of Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012, diagnostic protocols were quickly published and deployed globally. Objectives: We set out to assess the quality of MERS-CoV molecular diagnostics worldwide. Study design: Both sensitivity and specificity were assessed using 12 samples containing different viral loads of MERS-CoV or common coronaviruses (OC43, 229E, NL63, HKU1). Results: The panel was sent to more than 106 participants, of which 99 laboratories from 6 continents returned 189 panel results. Scores ranged from 100% (84 laboratories) to 33% (1 laboratory). 15% of respondents reported quantitative results, 61% semi-quantitative (Ct-values or time to positivity) and 24% reported qualitative results. The major specific technique used was real-time RT-PCR using the WHO recommended targets upE, ORF1a and ORF1b. The evaluation confirmed that RT-PCRs targeting the ORF1b are less sensitive, and therefore not advised for primary diagnostics. Conclusions: The first external quality assessment MERS-CoV panel gives a good insight in molecular diagnostic techniques and their performances for sensitive and specific detection of MERS-CoV RNA globally. Overall, all laboratories were capable of detecting MERS-CoV with some differences in sensitivity. The observation that 8% of laboratories reported false MERS-CoV positive single assay results shows room for improvement, and the importance of using confirmatory targets. (C) 2015 The Authors. Published by Elsevier B.V.
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