期刊
BRAIN
卷 132, 期 -, 页码 1523-1535出版社
OXFORD UNIV PRESS
DOI: 10.1093/brain/awp048
关键词
hypothalamochiasmatic astrocytomas; molecular profile; proliferation; invasion; radial glia
资金
- Aventis, INCA
- Federation Nationale Enfant et Sante
- Societe Francaise de Lutte contre les Cancers et les leucemies de l'Enfant et de l'adolescent
- Amelie la vie
- ARTC Sud
- INCA
Pilocytic astrocytomas are WHO grade I gliomas that occur predominantly in childhood. They share features of both astroglial and oligodendroglial lineages. These tumours affect preferentially the cerebellum (benign clinical course) and the optic pathway, especially the hypothalamochiasmatic region (poor prognosis). Understanding the molecular basis responsible for the aggressive behaviour of hypothalamochiasmatic pilocytic astrocytomas is a prerequisite to setting up new molecular targeted therapies. We used the microarray technique to compare the transcriptional profiles of five hypothalamochiasmatic and six cerebellar pilocytic astrocytomas. Validation of the microarray results and comparison of the tumours with normal developing tissue was done by quantitative real-time PCR and immunohistochemistry. Results demonstrate that cerebellar and hypothalamochiasmatic pilocytic astrocytomas are two genetically distinct and topography-dependent entities. Numerous genes upregulated in hypothalamochiasmatic pilocytic astrocytomas also increased in the developing chiasm, suggesting that developmental genes mirror the cell of origin whereas migrative, adhesive and proliferative genes reflect infiltrative properties of these tumours. Of particular interest, NOTCH2, a gene expressed in radial glia and involved in gliomagenesis, was upregulated in hypothalamochiasmatic pilocytic astrocytomas. In order to find progenitor cells that could give rise to hypothalamo-chiasmatic pilocytic astrocytomas, we performed a morphological study of the hypothalamochiasmatic region and identified, in the floor of the third ventricle, a unique population of vimentin- and glial fibrillary acidic protein-positive cells highly suggestive of radial glia cells. Therefore, pilocytic astrocytomas of the hypothalamochiasmatic region should be considered as a distinct entity which probably originates from a unique population of cells with radial glia phenotype.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据