期刊
VASCULAR HEALTH AND RISK MANAGEMENT
卷 7, 期 -, 页码 425-431出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/VHRM.S22435
关键词
shear stress; African American; NADPH oxidase; HUVECs; oxidative stress
资金
- NIH/NHLBI [RO1 HL085497]
- NIH/NIA [KO1AG019640]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL085497] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [K01AG019640] Funding Source: NIH RePORTER
Background: African American ethnicity is an independent risk factor for exaggerated oxidative stress, which is related to inflammation, hypertension, and cardiovascular disease. Recently, we reported that in vitro oxidative stress and inflammation levels differ between African American and Caucasian human umbilical vein endothelial cells (HUVECs), African American HUVECs having higher levels of both. However, it remains to be shown whether the cells would respond differently to external stimuli. Methods: We used a cone and plate viscometer to apply laminar shear stress (LSS) as an aerobic exercise mimetic to compare the responses by race. HUVECs were exposed to static conditions (no LSS), low LSS (5 dyne/cm(2)), and moderate LSS (20 dyne/cm(2)). Results: It was found that African American HUVECs had higher levels of oxidative stress under static conditions, and when LSS was applied protein expression levels (NADPH oxidase NOX2, NOX4 and p47phox subunits, eNOS, SOD2, and catalase) and biomarkers (NO, SOD, and total antioxidant capacity) were modulated to similar levels between race. Conclusion: African American HUVECs may be more responsive to LSS stimulus indicating that aerobic exercise prescriptions may be valuable for this population since the potential exists for large in vivo improvements in oxidative stress levels along the endothelial layer in response to increased shear flow.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据