期刊
BONE MARROW TRANSPLANTATION
卷 45, 期 2, 页码 379-384出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/bmt.2009.140
关键词
regulatory T cells; rapamycin; graft-versus-host disease
资金
- NCI NIH HHS [P01 CA047741] Funding Source: Medline
Rapamycin (RAPA) is an immunosuppressive drug that prevents and treats graft-versus-host disease (GVHD) after allogeneic hematopoietic cell transplant (HCT). One possible mechanism for its efficacy is induction of tolerance, through increased number or enhanced survival of regulatory T cells. In our experiments, B10.D2 BM and splenocytes were injected into lethally irradiated BALB/cJ recipients. The mice received i.p. injections of either RAPA or vehicle control on days 1-28. There was a significant survival advantage in RAPA-treated mice. Evaluation of the skin biopsies showed a dense cellular infiltrate in RAPA-treated mice. Further characterization of these cells revealed a higher percentage of regulatory T cells characterized by FoxP3-positive cells in high-dose RAPA-treated mice as compared with controls on day 30. This effect appears to be dose dependent. When peripheral blood analysis for FoxP3-positive cells was performed, there was no significant difference observed in the RAPA-treated mice as compared with control mice. These data show a novel mechanism of rapamycin in GVHD, accumulation of regulatory T cells in the GVHD target tissue: the skin. Bone Marrow Transplantation (2010) 45, 379-384; doi:10.1038/bmt.2009.140; published online 13 July 2009
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